ISOLATION AND ANALYSIS OF A HUMAN CDNA HIGHLY HOMOLOGOUS TO THE YEAST GENE ENCODING L17A RIBOSOMAL-PROTEIN

A cDNA from human brain poly(A)+RNA with significant similarity to the gene encoding yeast L17A large subunit ribosomal (r) protein (L17A) was isolated using the polymerase chain reaction. The deduced amino acid (aa) sequence of 140 aa (calculated pI of 10.79) exhibits a 78% similarity to that of the yeast L17A r protein (88% when conservative aa replacements are considered as well). This indicates that L17A is one of the best conserved r-proteins and therefore may play a critical role in ribosome function. In contrast to its eubacterial and chloroplast counterparts, human L17A contains an N-terminal extension of 19 aa which may be involved in nuclear targeting of the r-protein. Approximately five to seven genes in mammalian genomes give strong hybridization signals when probed with the human L17A homologue cDNA. Whereas the L17A homologue was found to be expressed at similar levels in several human tissues as a transcript of 600 nucleotides, a several-fold higher transcript level was detected in the rapidly growing neuroblastoma cell line, SK-N-BE.