THE EFFECT OF AQUEOUS SURFACTANT SOLUTIONS ON ALCOHOL-DEHYDROGENASE (LADH)

被引：30

作者：

CREAGH, AL ^{[1
]}

PRAUSNITZ, JM ^{[1
]}

BLANCH, HW ^{[1
]}

机构：

[1] UNIV CALIF BERKELEY,DEPT CHEM ENGN,BERKELEY,CA 94720

来源：

BIOTECHNOLOGY AND BIOENGINEERING | 1993年 / 41卷 / 01期

关键词：

LADH; SURFACTANTS; EPR; CD; FLUORESCENCE;

D O I：

10.1002/bit.260410120

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Alcohol dehydrogenase (LADH) was studied in aqueous solutions of surfactants to determine its structural and catalytic characteristics. Fluorescence, circular dichroism (CD), and electron paramagnetic resonance (EPR) techniques were used to study structural changes to the enzyme. The activity of LADH in catalyzing the oxidation of ethanol was investigated. Short-chain alkyl sulfonates and sulfates did not deactivate LADH or alter its structure. Longer and branched alkyl sulfates and sulfonates, as well as a cationic surfactant (CTAB), affected both LADH activity and conformation.

引用

页码：156 / 161

页数：6

共 18 条

[1] ADAMSON AW, 1982, PHYSICAL CHEM SURFAC
[2] Berliner Lj, 1976, SEELING SPIN LABELLI
[3] EVIDENCE FOR DISSOCIATION OF HORSE LIVER ALCOHOL DEHYDROGENASE INTO SUBUNITS IN PRESENCE OF DETERGENT
BLOMQUIST, CH
SMITH, DA
MARTINEZ, AM
[J]. ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1967, 122 (01) : 248 - +
[4] Campbell I.D., 1984, BIOL SPECTROSCOPY
[5] STRUCTURE AND FRACTAL DIMENSION OF PROTEIN-DETERGENT COMPLEXES
CHEN, SH
TEIXEIRA, J
[J]. PHYSICAL REVIEW LETTERS, 1986, 57 (20) : 2583 - 2586
[6] CREAGH AL, 1992, UNPUB ENZ MICROB TEC
[7] THE STRUCTURE AND THERMODYNAMICS OF PROTEIN-SDS COMPLEXES IN SOLUTION AND THE MECHANISM OF THEIR TRANSPORTS IN GEL-ELECTROPHORESIS PROCESS
GUO, XH
CHEN, SH
[J]. CHEMICAL PHYSICS, 1990, 149 (1-2) : 129 - 139
[8] SMALL-ANGLE NEUTRON-SCATTERING STUDY OF THE STRUCTURE OF PROTEIN DETERGENT COMPLEXES
GUO, XH
ZHAO, NM
CHEN, SH
TEIXEIRA, J
[J]. BIOPOLYMERS, 1990, 29 (02) : 335 - 346
[9] FLUORESCENCE CHANGES ASSOCIATED WITH DENATURATION OF ALCOHOL DEHYDROGENASE
HEITZ, JR
BRAND, L
[J]. ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1971, 144 (01) : 286 - &
[10] LUNDAHL P, 1986, BIOCHIM BIOPHYS ACTA, V873, P248

← 1 2 →