5'-UPSTREAM CIS-ELEMENTS AND BINDING FACTOR(S) POTENTIALLY INVOLVED IN LIGHT-REGULATED EXPRESSION OF A BRASSICA-NAPUS RBCS GENE

被引:11
作者
FIEBIG, C [1 ]
LINK, G [1 ]
机构
[1] RUHR UNIV BOCHUM,UNIV STR 150,W-4630 BOCHUM,GERMANY
关键词
DNA-PROTEIN INTERACTION; PLANT NUCLEAR GENE PROMOTER; PHOTOREGULATION; RUBISCO (RIBULOSE-1,5-BISPHOSPHATE CARBOXYLASE OXYGENASE); TRANSCRIPTION FACTOR;
D O I
10.1007/BF00318476
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
The 5'-upstream region, of a Brassica napus rbcS gene contains sequence elements that resemble the cis-regulatory elements described for other species. In band shift competition assays using whole-cell extracts and sets of deletion fragments we could assign protein binding to a region located 110-130 base pairs upstream of the transcription start site. DNase I protection experiments showed that the binding region is non-contiguous and reveals symmetry. On the non-coding strand, the sequence contains 5'-CAC-3' and 5'-GTGG-3' elements which together resemble the contiguous G-box core motif, CACGTGG, of other rbcS genes. The DNA-protein interaction was more efficient with extracts from light-grown than with those from dark-grown seedlings, indicating the possible involvement of light-regulated factor(s).
引用
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页码:161 / 168
页数:8
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