STUDIES ON THE ABILITY OF MINOR-GROOVE BINDERS TO INDUCE SUPERCOILING IN DNA

被引：33

作者：

STORL, K ^{[1
]}

BURCKHARDT, G ^{[1
]}

LOWN, JW ^{[1
]}

ZIMMER, C ^{[1
]}

机构：

[1] UNIV ALBERTA,DEPT CHEM,EDMONTON T6G 2E1,ALBERTA,CANADA

来源：

FEBS LETTERS | 1993年 / 334卷 / 01期

关键词：

MINOR GROOVE BINDER; PLASMID DNA; SUPERCOILING;

D O I：

10.1016/0014-5793(93)81678-S

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The effect of various non-intercalating minor groove binders on closed circular DNA in the presence of topoisomerase I has been studied by means of agarose gel electrophoresis. Analogues of the netropsin series (lexitropsins) and SN-6999 can effectively produce positive supercoils, as indicated by analysis of the topoisomers in the presence of chloroquine and the evaluated linking number changes. Analogues of the distamycin series are less effective, and bisquaternary ammonium heterocycles, as well as DAPI and pentamidine, were found to be ineffective ligands. The large differences observed in the ability of minor groove binders to induce positive supercoils are discussed.

引用

页码：49 / 54

页数：6

共 31 条

[1]

BAGULEY BC, 1982, MOL CELL BIOCHEM, V43, P167

[2]

BAUER W, 1990, LANDOLTBORNSTEIN, V1

[3] NETROPSIN AND BIS-NETROPSIN ANALOGS AS INHIBITORS OF THE CATALYTIC ACTIVITY OF MAMMALIAN DNA TOPOISOMERASE-II AND TOPOISOMERASE CLEAVABLE COMPLEXES [J].