LONG-TERM CULTIVATION OF LYMPH NODE FRAGMENTS AND CELLS OF HYPERIMMUNIZED RABBITS

被引：5

作者：

SURJAN, M

SURJAN, L

机构：

[1] State Institute of Hygiene, Budapest

来源：

LIFE SCIENCES PART 1 PHYSIOLOGY AND PHARMACOLOGY AND PART 2 BIOCHEMISTRY GENERAL AND MOLECULAR BIOLOGY | 1968年 / 7卷 / 18P2期

关键词：

D O I：

10.1016/0024-3205(68)90174-4

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

1. Lymph node and spleen fragments from hyperimmunized rabbits were cultivated in Parker 199 medium containing 0.5 U insulin, 0.01 Lf unit tetanus toxoid, 0.01 or 0.001 ml phytohemagglutinin and in some cases 10% normal rabbit serum. The fragments produced antibody for 5-8 weeks. 2. Lymph node or spleen cells embedded in 2% agar could produce antibody for 3-5 weeks even in the absence of serum. 3. Antibody titration was made by hemagglutination. The sensitivity of the method was increased by using horse instead of sheep erythrocytes. © 1968.

引用

页码：975 / &

共 13 条

[1] FORMATION OF SPECIFIC ANTIBODIES AND GAMMA-GLOBULIN INVITRO - STUDY OF THE SYNTHETIC ABILITY OF VARIOUS TISSUES FROM RABBITS IMMUNIZED BY DIFFERENT METHODS [J].