RECOMBINANT HUMAN-MOUSE CHIMERIC MONOCLONAL-ANTIBODY SPECIFIC FOR HUMAN ADENOCARCINOMA ASSOCIATED ANTIGEN

被引：6

作者：

NAKASHIMA, M ^{[1
]}

NISHIMURA, Y ^{[1
]}

WATANABE, T ^{[1
]}

机构：

[1] KYUSHU UNIV,MED INST BIOREGULAT,DEPT MOLEC IMMUNOL,MAIDASHI 3-1-1,HIGASHI KU,FUKUOKA 812,JAPAN

来源：

HYBRIDOMA | 1991年 / 10卷 / 01期

关键词：

D O I：

10.1089/hyb.1991.10.1

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We have recently described a class-switched (IgM to IgG1) human-mouse chimeric antibody. In the present study, a human-mouse chimeric antibody specific for human adenocarcinoma-associated antigen YH206 antigen was constructed by fusing murine variable region genes (V-kappa and V(H)) to human constant region (gamma-1,kappa). The murine variable domain genes were isolated from a functional murine hybridoma cell line, YH206, which secreted IgM monoclonal antibody specific for YH206 antigen. The fusion genes of heavy and light chains were introduced into the immunoglobulin non-producing mouse myeloma cell line X63-Ag8.653 by electroporation. We obtained transformants which secreted class-switched human-mouse chimeric antibodies specific for YH206 antigen. A dot immunobinding assay demonstrated that the class-switched chimeric antibody retained the ability to bind to the YH206 antigen.

引用

页码：1 / 9

页数：9

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