GAS-LIQUID-CHROMATOGRAPHY OF ISOBUTYL ESTER, N(O)-HEPTAFLUOROBUTYRATE DERIVATIVES OF AMINO-ACIDS ON A GLASS CAPILLARY COLUMN FOR QUANTITATIVE SEPARATION IN CLINICAL BIOLOGY

被引：67

作者：

DESGRES, J ^{[1
]}

BOISSON, D ^{[1
]}

PADIEU, P ^{[1
]}

机构：

[1] CTR HOSP REG UNIV,EXPLORAT FONCT & CHIM BIOL LAB,F-21034 DIJON,FRANCE

来源：

JOURNAL OF CHROMATOGRAPHY | 1979年 / 162卷 / 02期

关键词：

D O I：

10.1016/S0378-4347(00)81907-6

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A gas chromatographic method adapted to routine analysis has been developed for quantitative separation on glass capillary columns for free proteic and other known amino acids normally or abnormally found in physiological fluids. The procedure involves ion-exchange chromatography and isobutyl ester, N(O)-heptafluorobutyrate derivatization of free plasma and urine amino acid samples. Derivatized components were ascertained by combined gas chromatography-mass spectrometry. The use of glass for the capillary column is mandatory to achieve qualitative and quantitative analysis of the known occurring amino acids in urine and small plasma samples. Quantitative analysis of several types of human amino acid disorders are presented. © 1979.

引用

页码：133 / 152

页数：20

共 52 条

[1] ABRAMSON FP, 1974, ANAL BIOCHEM, V51, P482
[2] DETERMINATION OF AMINO-ACID PROFILES IN BIOLOGICAL SAMPLES BY GAS-CHROMATOGRAPHY
ADAMS, RF
[J]. JOURNAL OF CHROMATOGRAPHY, 1974, 95 (02): : 189 - 212
[3] QUANTITATIVE-ANALYSIS OF PROTEIN AND NON-PROTEIN AMINO-ACIDS BY GAS-LIQUID-CHROMATOGRAPHY
AMICO, V
ORIENTE, G
TRINGALI, C
[J]. JOURNAL OF CHROMATOGRAPHY, 1977, 131 (JAN21): : 233 - 238
[4] BARBIER F, 1972, AMINOACIDOPATHIES J, P157
[5] BARBIER F, 1974, MASS SPECTROMETRY BI, P119
[6] BARBIERCHAPUIS F, UNPUBLISHED
[7] Bayer E., 1958, GAS CHROMATOGRAPHY, P333
[8] BREAKDOWN OF TRIFLUOROACETYLATED ESTERS OF CYSTEINE AND HYDROXYL AMINO ACIDS DURING GAS CHROMATOGRAPHY - EFFECTS CAUSED BY SOME POLAR STATIONARY PHASES
DARBRE, A
BLAU, K
[J]. BIOCHIMICA ET BIOPHYSICA ACTA, 1966, 126 (03) : 591 - &
[9] GAS CHROMATOGRAPHY OF VOLATILE AMINO ACID DERIVATIVES .4. MIXED STATIONARY PHASES FOR SEPARATION OF N-TRIFLUOROACETYLATED AMINO ACID N-AMYL ESTERS
DARBRE, A
BLAU, K
[J]. JOURNAL OF CHROMATOGRAPHY, 1967, 29 (01): : 49 - &
[10] GAS-LIQUID CHROMATOGRAPHY OF RADIOACTIVE AMINO ACIDS AS THEIR TRIFLUOROACETYL ESTER DERIVATIVES
DELFAVERO, A
DARBRE, A
WATERFIELD, M
[J]. JOURNAL OF CHROMATOGRAPHY, 1969, 40 (02): : 213 - +

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