RAPID ASSAY FOR THE SIMULTANEOUS DETERMINATION OF RESIDUES OF OXOLINIC ACID AND FLUMEQUINE IN FISH-TISSUES BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引：24

作者：

STEFFENAK, I

HORMAZABAL, V

YNDESTAD, M

机构：

[1] Department of Food Hygiene, Norwegian College of Veterinary Medicine, N-0033, Oslo 1

来源：

JOURNAL OF LIQUID CHROMATOGRAPHY | 1991年 / 14卷 / 01期

关键词：

D O I：

10.1080/01483919108049597

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A simple and rapid method for the simultaneous extraction and determination of residues of oxolinic acid (OX) and flumequine (FQ) in fish tissue, muscle and liver, is presented. The samples were extracted with acetonitrile and ammonia. The drugs were then extracted into a water phase, acidified, and extracted with CHCl3. After evaporation, the sample was redissolved in mobile phase and filtered through a spin-X centrifuge filter. The filtrate was injected onto the HPLC. The calibration curves were linear, and the recovery of oxolinic acid was 101-104 % while the recovery of flumequine was 88-94 %. The detection limits were 5 ng/g for oxolinic acid and 10 ng/g for flumequine.

引用

页码：61 / 70

页数：10

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