DETECTION OF SPECIFIC ANTIGENS IN THE VESICULAR-ARBUSCULAR MYCORRHIZAL FUNGI GIGASPORA-MARGARITA AND ACAULOSPORA-LAEVIS USING POLYCLONAL ANTIBODIES TO SOLUBLE SPORE FRACTIONS

被引：15

作者：

SANDERS, IR ^{[1
]}

RAVOLANIRINA, F ^{[1
]}

GIANINAZZIPEARSON, V ^{[1
]}

GIANINAZZI, S ^{[1
]}

LEMOINE, MC ^{[1
]}

机构：

[1] INRA,PHYTOPARASITOL LAB,GENET & AMELIORAT PLANTES STN,CNRS,BV 1540,F-21034 DIJON,FRANCE

来源：

MYCOLOGICAL RESEARCH | 1992年 / 96卷

关键词：

D O I：

10.1016/S0953-7562(09)81093-9

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Polyclonal antibodies raised against soluble extracts of spores of Acaulospora laevis and Gigaspora margarita only cross-reacted in a dot immunobinding assay (DIBA) with heterologous antigens from spores of some of the VAM fungi tested. Major cross-reactivity was between A. laevis and Gig. margarita. Ammonium sulphate precipitation of protein antigens from extracts of these two fungi, or their treatment with periodate, rendered the activity of antibodies specific, indicating that cross-reactions between the fungal extracts in DIBA is probably due to the presence of non-specific glycoconjugates. Analysis of extracts by an indirect enzyme-linked immunosorbent assay (ELISA) gave more specific reactions, and immunoblotting of PAGE-separated extracts showed that homologous antisera reacted with different protein bands in the two fungi.

引用

页码：477 / 480

页数：4

共 22 条

[1] ENZYME-LINKED IMMUNOSORBENT-ASSAY (ELISA) TO IDENTIFY ENDOMYCORRHIZAL FUNGI
ALDWELL, FEB
HALL, IR
SMITH, JMB
[J]. SOIL BIOLOGY & BIOCHEMISTRY, 1983, 15 (03) : 377 - 378
[2] ENZYME-LINKED IMMUNOSORBENT-ASSAY AS AN AID TO TAXONOMY OF THE ENDOGONACEAE
ALDWELL, FEB
HALL, IR
SMITH, JMB
[J]. TRANSACTIONS OF THE BRITISH MYCOLOGICAL SOCIETY, 1985, 84 (MAY): : 399 - 402
[3] BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[4] MONOCLONAL-ANTIBODIES TO ANTIGENS IN THE PERIBACTEROID MEMBRANE FROM RHIZOBIUM-INDUCED ROOT-NODULES OF PEA CROSS-REACT WITH PLASMA-MEMBRANES AND GOLGI BODIES
BREWIN, NJ
ROBERTSON, JG
WOOD, EA
WELLS, B
LARKINS, AP
GALFRE, G
BUTCHER, GW
[J]. EMBO JOURNAL, 1985, 4 (03) : 605 - 611
[5] BURGERMEISTER W, 1984, PHYTOPATHOL Z, V111, P15
[6] Clark M.F., 1984, METHODS VIROLOGY, P51, DOI DOI 10.1016/B978-0-12-470207-3.50009-7
[7] DISC ELECTROPHORESIS .2. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS
DAVIS, BJ
[J]. ANNALS OF THE NEW YORK ACADEMY OF SCIENCES, 1964, 121 (A2) : 404 - &
[8] EFFICACY OF ENDOMYCORRHIZAL-FUNGUS ISOLATES AND INOCULUM QUANTITIES REQUIRED FOR GROWTH-RESPONSE
HAAS, JH
KRIKUN, J
[J]. NEW PHYTOLOGIST, 1985, 100 (04) : 613 - 621
[9] METHODS FOR REDUCING NONSPECIFIC ANTIBODY-BINDING IN ENZYME-LINKED IMMUNOSORBENT ASSAYS
KENNA, JG
MAJOR, GN
WILLIAMS, RS
[J]. JOURNAL OF IMMUNOLOGICAL METHODS, 1985, 85 (02) : 409 - 419
[10] USE OF THE INDIRECT IMMUNOFLUORESCENT TECHNIQUE TO STUDY THE VESICULAR-ARBUSCULAR FUNGUS GLOMUS-EPIGAEUM AND OTHER GLOMUS SPECIES
KOUGH, J
MALAJCZUK, N
LINDERMAN, RG
[J]. NEW PHYTOLOGIST, 1983, 94 (01) : 57 - 62

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