THE MOUSE LOW-DENSITY-LIPOPROTEIN RECEPTOR GENE - CDNA SEQUENCE AND EXON-INTRON STRUCTURE

被引：34

作者：

HOFFER, MJV

VANECK, MM

PETRIJ, F

VANDERZEE, A

DEWIT, E

MEIJER, D

GROSVELD, G

HAVEKES, LM

HOFKER, MH

FRANTS, RR

机构：

[1] LEIDEN UNIV, DEPT HUMAN GENET, MGC, POB 9503, 2300 RA LEIDEN, NETHERLANDS

[2] TNO, IVVO, GAUBIUS LAB, 2300 AK LEIDEN, NETHERLANDS

[3] ERASMUS UNIV, DEPT CELL BIOL, 3000 DR ROTTERDAM, NETHERLANDS

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1993年 / 191卷 / 03期

关键词：

D O I：

10.1006/bbrc.1993.1299

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The low density lipoprotein (LDL) receptor plays a central role in the cholesterol metabolism. The cDNA sequence of the mouse low density lipoprotein receptor (Ldlr) gene has been determined and shows 76% homology with the human gene. The exon-intron structure has been determined for the 129/J mouse strain. The gene is composed of 18 exons and spans a region of 28 kb. In addition, the promoter regions of the mouse and human genes are homologous. Northern blot analysis revealed an mRNA of approximately 5 kb. The cloning of the Ldlr gene will enhance the usefulness of the mouse for the study of cholesterol metabolism and, in particular, for carrying out gene targeting experiments. © 1993 Academic Press. All rights reserved.

引用

页码：880 / 886

页数：7

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