DIRECT SPECTROPHOTOMETRIC ESTIMATION OF FERROCYANIDE AND ITS POSSIBLE USES IN SULFHYDRIL OXIIDATION STUDIES

被引：15

作者：

KIDBY, DK

机构：

[1] Sub-department of Chemical Microbiology, Department of Biochemistry, University of Cambridge, England

来源：

ANALYTICAL BIOCHEMISTRY | 1969年 / 28卷 / 1-3期

关键词：

D O I：

10.1016/0003-2697(69)90174-2

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

1. 1. The direct spectrophotometry of ferrocyanide in the presence of ferricyanide has been examined. 2. 2. Ferrocyanide at a concentration of 1 × 10-5 M is easily measurable in the presence of a 50-fold excess of ferricaynide. 3. 3. The measurement may be performed over a wide range of conditions, including those suited to the oxidation of most biological SH groups. 4. 4. The method has been compared with the Prussian blue method for the estimation of oxidation of the SH group of cysteine. 5. 5. The method has many advantages over the Prussian blue method, including its possible use in direct kinetic measurements. © 1969.

引用

页码：230 / &

共 13 条

[1] The sulfhydryl groups of egg albumin [J].