SMALL-SCALE TECHNIQUES FOR THE ANALYSIS OF RECOMBINANT PLASMIDS

被引：17

作者：

HEPBURN, AG ^{[1
]}

HINDLEY, J ^{[1
]}

机构：

[1] UNIV BRISTOL,SCH MED,DEPT BIOCHEM,BRISTOL BS8 1TD,AVON,ENGLAND

来源：

JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS | 1979年 / 1卷 / 05期

关键词：

Agrobacterium; cloning; recombinant DNA; tumorigenic DNA;

D O I：

10.1016/0165-022X(79)90004-6

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Using the cloning of part of the T-DNA of pTiC58 from Agrobacterium tumefaciens as an example, techniques are described which enable recombinant plasmids to be mapped and used as hybridization probes. In all cases the starting material is a colony of cells grown on an agar plate which is then subjected to lysis by lysozyme and Triton X-100 in volumes of the order of 300 μl thus eliminating the need for handling and centrifuging liquid cultures under restrictive containment conditions. © 1979.

引用

页码：299 / 308

页数：10

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