ISOLATION OF THE GOLGI-APPARATUS FROM SUSPENSION CULTURED TOBACCO CELLS AND PRELIMINARY-OBSERVATIONS ON THE INTRACELLULAR-TRANSPORT OF EXTENSIN-PRECURSOR

Suspension cultured tobacco cells were fractionated into 3 fractions by sucrose density gradient centrifugation. The Golgi stacks were recovered over the density range of 1.15 to 1.17 g·cm-3. The contents of the fractions were identified by electron microscopic observations, phosphotungstic acid-chromic acid staining, and assays of marker enzymes or organella. Cells were pulse labelled with14C-proline, and changes in content of14C-hydroxyproline-containing macromolecules in the fractions were followed. It was demonstrated that extensin-precursor was transported from the Golgi apparatus to the cell wall, and participation of a vesicular structure in the transport was suggested. © 1979 The Botanical Society of Japan.