DISTRIBUTION OF GONADOTROPIN-RELEASING HORMONE-LIKE PEPTIDES IN THE BRAIN DURING DEVELOPMENT OF JUVENILE MALE RANA-ESCULENTA

被引:14
作者
DANIELLO, B
MASUCCI, M
DIMEGLIO, M
CIARCIA, G
RASTOGI, RK
机构
[1] NAPLES UNIV, DEPT ZOOL, VIA MEZZOCANNONE 8, I-80134 NAPLES, ITALY
[2] NAPLES UNIV, DEPT EVOLUT & COMPARAT BIOL, I-80134 NAPLES, ITALY
关键词
BRAIN; GONADOTROPIN-RELEASING HORMONE; SPERMATOGENESIS; REPRODUCTIVE CYCLE; IMMUNOHISTOCHEMISTRY; DEVELOPMENT; ONTOGENIC; RANA-ESCULENTA (ANURA);
D O I
10.1007/BF00318138
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
The distribution and density of cell bodies and fibers immunoreactive to GnRH-like peptides were investigated in the brain of male juvenile frogs (Rana esculenta) during postmetamorphic development. An immunohistochemical technique was used, involving antisera raised against 4 variants of GnRH: mammalian GnRH, chicken GnRH-I, chicken GnRH-II and salmon GnRH. A comparison of the immunohistochemical distribution at 8 different developmental stages shows that the maximum density of immunoreactive-GnRH elements, and the full distributional complexity of this system, is attained at the completion of spermatogenesis. Immunoreactive-GnRH cell bodies first appear in the anterior preoptic area during the metamorphic climax, and then appear sequentially in the medial septal area, tegmentum and, lastly, in the retrochiasmatic area and olfactory bulb when immunoreactive-fibers also reach the cerebellum. The GnRH system reacts positively to antisera for all 4 GnRH variants, but immunoreactivity for chicken GnRH-I is the weakest.
引用
收藏
页码:51 / 55
页数:5
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