A PLASMID-ENCODED SURFACE PROTEIN FOUND IN CHRONIC-DISEASE ISOLATES OF COXIELLA-BURNETII

被引:16
作者
MINNICK, MF
HEINZEN, RA
RESCHKE, DK
FRAZIER, ME
MALLAVIA, LP
机构
[1] WASHINGTON STATE UNIV, DEPT MICROBIOL, PULLMAN, WA 99164 USA
[2] PACIFIC NW LAB, DEPT BIOL, RICHLAND, WA 99352 USA
关键词
D O I
10.1128/IAI.59.12.4735-4739.1991
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
The cbbE' gene codes for the E' protein of Coxiella burnetii and was detected in genomic DNA from all known human isolates of the biotzere strain but not in DNA from the other five strains of C. burnetii. The biotzere strain is strictly associated with chronic disease in humans. Extrinsic iodination of biotzere strain cells radiolabeled a 55-kDa protein which comigrated on sodium dodecyl sulfate-polyacrylamide gel electrophoresis with the E' protein synthesized in vitro from recombinants containing the cbbE' gene. The I-125-labeled 55-kDa protein was immunoprecipitated with polyclonal anti-E' antiserum, confirming its identity as E'. Predicted secondary structure of the E' polypeptide shows six regions of beta-sheet structure and an alpha-helix near the C terminus with adequate lengths to span a membrane. The predicted hydropathy profile of E' is similar to profiles of known outer membrane proteins and corroborates the biochemical data, indicating that the protein is located in the outer membrane of C. burnetii.
引用
收藏
页码:4735 / 4739
页数:5
相关论文
共 36 条
[1]   STRUCTURAL REQUIREMENTS OF A MEMBRANE-SPANNING DOMAIN FOR PROTEIN ANCHORING AND CELL-SURFACE TRANSPORT [J].
ADAMS, GA ;
ROSE, JK .
CELL, 1985, 41 (03) :1007-1015
[2]  
Ausubel FM., 1988, CURRENT PROTOCOLS MO
[3]  
CHOU PY, 1978, J MOL BIOL, V120, P45
[4]   A COMPREHENSIVE SET OF SEQUENCE-ANALYSIS PROGRAMS FOR THE VAX [J].
DEVEREUX, J ;
HAEBERLI, P ;
SMITHIES, O .
NUCLEIC ACIDS RESEARCH, 1984, 12 (01) :387-395
[5]   INDUCTION OF HEPATITIS-A VIRUS-NEUTRALIZING ANTIBODY BY A VIRUS-SPECIFIC SYNTHETIC PEPTIDE [J].
EMINI, EA ;
HUGHES, JV ;
PERLOW, DS ;
BOGER, J .
JOURNAL OF VIROLOGY, 1985, 55 (03) :836-839
[6]  
GILMAN AG, 1980, PHARMACOL BASIS THER, P1092
[7]   PLASMID-ASSOCIATED VIRULENCE OF SALMONELLA-TYPHIMURIUM [J].
GULIG, PA ;
CURTISS, R .
INFECTION AND IMMUNITY, 1987, 55 (12) :2891-2901
[8]   THE TOLC PROTEIN OF ESCHERICHIA-COLI-K12 IS SYNTHESIZED IN A PRECURSOR FORM [J].
HACKETT, J ;
MISRA, R ;
REEVES, P .
FEBS LETTERS, 1983, 156 (02) :307-310
[9]   ANTIGENIC VARIATION IN THE PHASE-I LIPOPOLYSACCHARIDE OF COXIELLA-BURNETII ISOLATES [J].
HACKSTADT, T .
INFECTION AND IMMUNITY, 1986, 52 (01) :337-340
[10]   NATURAL-HISTORY OF ORAL SALMONELLA-DUBLIN INFECTION IN BALB/C MICE - EFFECT OF AN 80-KILOBASE-PAIR PLASMID ON VIRULENCE [J].
HEFFERNAN, EJ ;
FIERER, J ;
CHIKAMI, G ;
GUINEY, D .
JOURNAL OF INFECTIOUS DISEASES, 1987, 155 (06) :1254-1259