KINETICS OF OVINE INTERFERON-GAMMA PRODUCTION - DETECTION OF MESSENGER-RNA AND CHARACTERIZATION OF BIOLOGICAL-ACTIVITY

被引：21

作者：

ENTRICAN, G ^{[1
]}

MCINNES, CJ ^{[1
]}

ROTHEL, JS ^{[1
]}

HAIG, DM ^{[1
]}

机构：

[1] CSIRO,DIV ANIM HLTH,PARKVILLE,VIC 3052,AUSTRALIA

来源：

VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY | 1992年 / 33卷 / 1-2期

关键词：

D O I：

10.1016/0165-2427(92)90044-Q

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The kinetics of interferon-gamma (IFN-gamma) production were studied in sheep mesenteric lymph node (MLN) cells at the molecular level using an ovine IFN-gamma cDNA probe and by bioassay which was verified by blocking antiviral activity with a monoclonal antibody (Mab) against recombinant bovine IFN-gamma IFN-gamma mRNA appeared in MLN cells within 4 h of stimulation with phorbol ester and Concanavalin A and was not detectable by 72 h after stimulation. Biologically active IFN-gamma appeared in the culture supernatants 8 h after stimulation and was still present 96 h later when de novo synthesis had terminated. Acid dialysis and Mab neutralisation demonstrated conclusively that native ovine IFN-gamma is a pH 2 labile cytokine.

引用

页码：171 / 178

页数：8

共 14 条

[1]

CERRETTI DP, 1986, J IMMUNOL, V136, P4561

[2]

DEMAEYER E, 1988, INTERFERONS OTHER RE, P174

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