HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF SUGARS IN AN INFUSION AND SOFT DRINKS USING A SILICA-BASED 3-MORPHOLINOPROPYL-BONDED STATIONARY PHASE

A recently developed column packing material, 3-morpholinopropylsilyl-modified silica gel (MPS), was successfully applied to the separation of many kinds of sugars (rhamnose, xylose, arabitol. glucose, mannose, sucrose, maltose, melibiose, raffinose and maltotriose) and the determination of glucose or/and sucrose in an infusion, drinks for sports and soft drinks, with elution with acetonitrile-10 mM imidazole-borate buffer (pH 6.5) (90: 10, v/v) by refractive index detection. The MPS column was found to be chemically stable under the elution conditions used and during continuous operation for over 500 h.