NUCLEAR-MAGNETIC-RESONANCE MEASUREMENT OF INTRACELLULAR SODIUM IN THE PERFUSED NORMOTENSIVE AND SPONTANEOUSLY HYPERTENSIVE RAT-HEART

被引：31

作者：

JELICKS, LA ^{[1
]}

GUPTA, RK ^{[1
]}

机构：

[1] YESHIVA UNIV ALBERT EINSTEIN COLL MED,DEPT PHYSIOL & BIOPHYS,BRONX,NY 10461

来源：

AMERICAN JOURNAL OF HYPERTENSION | 1994年 / 7卷 / 05期

关键词：

SPONTANEOUSLY HYPERTENSIVE RAT; SODIUM; BLOOD PRESSURE; CARDIAC PACING; NA-23; NMR;

D O I：

10.1093/ajh/7.5.429

中图分类号：

R6 [外科学];

学科分类号：

1002 ; 100210 ;

摘要：

We have used Na-23 nuclear magnetic resonance spectroscopy to examine the relationship between intracellular sodium and cardiac muscle alterations in genetic hypertension. In the spontaneously hypertensive rat (SHR) compared with the normotensive Wistar-Kyoto rat (WKY) (aged 15 to 19 weeks), mean systolic blood pressures (measured using the tail-cuff method) were significantly (P < .05) different (WKY: 118 +/- 8 mm Hg, n = 5; SHR: 185 a 9 mm Hg, n = 5). Heart weights were also increased significantly (P < .05) in SHR (grams dry heart to kilograms body weight ratio was 0.73 a 0.04, n 5, for SHR and 0.55 +/- 0.02, n = 5, for WKY). Intracellular sodium levels, measured using shift-reagent-aided and triple quantum filtered (TQF) nuclear magnetic resonance techniques, were significantly increased (P < .05) in the isolated Langendorff perfused hypertensive rat hearts (17.3 +/- 3.6 mmol/L, n = 5) compared with normotensive rat hearts (8.4 +/- 2.3 mmol/L, n = 5). These data demonstrate increased sodium in cardiac muscle in essential hypertension. We also investigated the effect of pacing on cardiac TQF Na-23 nuclear magnetic resonance and found an increase in TQF Na+ content in both WKY and SHR hearts on stepped up pacing. These results support the existence of sodium pump lag in the rat heart perfused at physiologic Ca2+ concentration and suggest that the hypertensive rat heart has adapted to compensate for increased basal intracellular Na+ and maintain a normal response to increased heart rate. Our data appear to suggest an ionic contribution to the cardiac hypertrophy of genetic hypertension in the rat.

引用

页码：429 / 435

页数：7

共 34 条

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