STUDIES OF COVALENTLY IMMOBILIZED PROTEIN MOLECULES BY SCANNING-TUNNELING-MICROSCOPY - THE ROLE OF WATER IN IMAGE-CONTRAST FORMATION

被引：32

作者：

LEGGETT, GJ

DAVIES, MC

JACKSON, DE

ROBERTS, CJ

TENDLER, SJB

WILLIAMS, PM

机构：

[1] VG SPM Laboratory for Biological Applications, Department of Pharmaceutical Sciences, University of Nottingham, Nottingham NG7 2RD, University Park

来源：

JOURNAL OF PHYSICAL CHEMISTRY | 1993年 / 97卷 / 35期

关键词：

D O I：

10.1021/j100137a006

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Protein molecules have been immobilized on gold surfaces using a novel covalent attachment technique. The naked molecules have been imaged with the scanning tunneling microscope (STM), and the effects of protein dehydration on STM image contrast have been investigated. A reversal of image contrast is observed upon reduction of the relative humidity inside the STM chamber from 33% to 5%. It is concluded that water plays a crucial role in the formation of the STM image of the protein molecules under ambient conditions.

引用

页码：8852 / 8854

页数：3

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