SYNTHESIS AND SECRETION OF CASEINS BY THE MOUSE MAMMARY-GLAND - PRODUCTION AND CHARACTERIZATION OF NEW POLYCLONAL ANTIBODIES

被引：18

作者：

BARASH, I

FAERMAN, A

PUZIS, R

PETERSON, D

SHANI, M

机构：

[1] Institute of Animal Science, ARO, the Volcani Center, Bet Dagan

来源：

MOLECULAR AND CELLULAR BIOCHEMISTRY | 1995年 / 144卷 / 02期

关键词：

CASEIN; ANTIBODIES; MAMMARY GLAND;

D O I：

10.1007/BF00944397

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Polyclonal antibodies to mouse alpha- and beta/gamma-caseins were raised in rabbits. These antibodies display tissue- and species specificity as shown by immunoblotting. Immunohistochemical analyses demonstrate that both alpha- and beta/gamma-caseins were synthesized and secreted from virtually all lactating mammary epithelial cells, in a pattern very similar to that of the mouse alpha-lactalbumin. Residual amounts of caseins were located also in the apical surface of epithelial cells surrounding the ducal lumen of virgin mammary gland sections. In contrast to the significant level of gamma-casein in the milk, the amount of this protein compared to alpha- or beta-caseins was extremely low in medium conditioned for 24 h by mammary explants of mid-pregnant mice immediately after explantation or after 4 days.

引用

页码：175 / 180

页数：6

共 15 条

[1] SYNTHESIS AND SECRETION OF HUMAN SERUM-ALBUMIN BY MAMMARY-GLAND EXPLANTS OF VIRGIN AND LACTATING TRANSGENIC MICE [J].