CHEMILUMINESCENT AND COLORIMETRIC DETECTION OF A FLUORESCEIN-LABELED PROBE AND A DIGOXIGENIN-LABELED PROBE AFTER A SINGLE HYBRIDIZATION STEP

被引:7
作者
CHENAL, V [1 ]
GRIFFAIS, R [1 ]
机构
[1] INST PASTEUR,RICKETTIALES & CHLAMYDIALES LAB,F-75724 PARIS,FRANCE
关键词
NONRADIOACTIVE PROBES; SINGLE HYBRIDIZATION STEP; DIGOXIGENIN; FLUORESCEIN; CHEMILUMINESCENCE; COLORIMETRY;
D O I
10.1006/mcpr.1994.1057
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
The objective of the simple and fast method we describe is the simultaneous hybridization of two non-radioactive probes and their detection from the same blot, using two different systems. These two probes are synthesized by PCR: one is label led with fluorescein and the other with digoxigenin. The former is detected by chemiluminescence and the latter by colorimetry. We applied this rapid and simple method to the specific detection of multiplex polymerase chain reaction products. We used the human herpes simplex virus HSV1 and HSV2 PCR models studied in our laboratory.
引用
收藏
页码:401 / 407
页数:7
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