EXPRESSION AND CDNA CLONING OF HUMAN HMGI-C PHOSPHOPROTEIN

被引：61

作者：

PATEL, UA

BANDIERA, A

MANFIOLETTI, G

GIANCOTTI, V

CHAU, KY

CRANEROBINSON, C

机构：

[1] UNIV PORTSMOUTH,BIOPHYS LABS,PORTSMOUTH PO1 2DT,ENGLAND

[2] UNIV TRIESTE,DIPARTIMENTO BIOCHIM BIOFIS & CHIM MACROMOLEC,I-34127 TRIESTE,ITALY

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1994年 / 201卷 / 01期

基金：

英国惠康基金;

关键词：

D O I：

10.1006/bbrc.1994.1669

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The HMGI family contains three members: I, Y and I-C. HMGI and HMGY are alternative splicings of the same gene and are essential transcription factors at several genetic loci. HMGI-G is transcribed from a different gene and is observed only in highly transformed cells. This work shows that human I-C is present in a more restricted range of cell types than I/Y and is absent from hemopoietic cells, as noted for mouse I-C. However, high expression in a human hepatoma line allowed the cloning of the cDNA and 812 bp of 5'-untranslated, 330 bp of coding and 58 bp of 3'-untranslated DNA were sequenced. The open reading frame showed 4 amino acid substitutions and one additional amino acid when compared to mouse I-C, none of them in the basic DNA binding motifs. (C) 1994 Academic Press, Inc.

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页码：63 / 70

页数：8

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