AUTOPHOSPHORYLATION AND ACTIVATION OF TRANSCRIPTIONAL ACTIVATOR PHOB OF ESCHERICHIA-COLI BY ACETYL PHOSPHATE IN-VITRO

被引：29

作者：

HIRATSU, K ^{[1
]}

NAKATA, A ^{[1
]}

SHINAGAWA, H ^{[1
]}

MAKINO, K ^{[1
]}

机构：

[1] OSAKA UNIV,MICROBIAL DIS RES INST,DEPT MOLEC MICROBIOL,SUITA,OSAKA 565,JAPAN

来源：

GENE | 1995年 / 161卷 / 01期

关键词：

PHOSPHATE REGULON; 2-COMPONENT REGULATORY SYSTEM; ACKA;

D O I：

10.1016/0378-1119(95)00259-9

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The PhoB protein, the transcriptional activator for the genes belonging to the phosphate regulon in Escherichia coli, was autophosphorylated in the presence of acetyl phosphate (acP) in vitro. The properties of phospho-PhoB, such as stability upon acid or alkali treatment and activating pstS transcription by RNA polymerase holoenzyme, were the same as those of phospho-PhoB produced from phospho-PhoR or phospho-PhoM. These results indicate that PhoB is an enzyme that catalyzes its own phosphorylation using acP, a low-molecular-weight metabolic intermediate.

引用

页码：7 / 10

页数：4

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