OXIDATIVE STRESS-RESPONSE IN YEAST - EFFECT OF GLUTATHIONE ON ADAPTATION TO HYDROGEN-PEROXIDE STRESS IN SACCHAROMYCES-CEREVISIAE

被引：223

作者：

IZAWA, S ^{[1
]}

INOUE, Y ^{[1
]}

KIMURA, A ^{[1
]}

机构：

[1] KYOTO UNIV,FOOD SCI RES INST,UJI,KYOTO 611,JAPAN

来源：

FEBS LETTERS | 1995年 / 368卷 / 01期

关键词：

GLUTATHIONE; L-BUTHIONINE SULFOXIMINE; 1-CHLORO-2,4-DINITROBENZENE; HYDROGEN PEROXIDE; SACCHAROMYCES CEREVISIAE;

D O I：

10.1016/0014-5793(95)00603-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Role of intracellular glutathione in the response of Saccharomyces cerevisiae to H2O2 was investigated. Depletion of cellular glutathione or inhibition of gamma-glutamylcysteine synthetase (GSH-I) enhanced the sensitivity to H2O2 and suppressed the adaptation to H2O2. A mutant deficient in GSH-I also showed the hypersensitivity and could not adapt to H2O2. Incubation of the cell with amino acids constituting glutathione (L-Glu, L-Cys, Gly) increased the intracellular glutathione content, and subsequently the cell acquired resistance against H2O2. These results strongly suggest that intracellular glutathione plays an important role in the adaptive response in S. cerevisiae to oxidative damage.

引用

页码：73 / 76

页数：4

共 35 条

[1] OXIDANTS, ANTIOXIDANTS, AND THE DEGENERATIVE DISEASES OF AGING [J].

AMES, BN ;

SHIGENAGA, MK ;

HAGEN, TM .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (17) :7915-7922

[2] INDUCIBILITY OF THE RESPONSE OF YEAST-CELLS TO PEROXIDE STRESS [J].

COLLINSON, LP ;

DAWES, IW .

JOURNAL OF GENERAL MICROBIOLOGY, 1992, 138 :329-335

[3]

DVIES JM, 1995, ARCH BIOCHEM BIOPHYS, V317, P1

[4]

FAHAY RC, 1983, FUNCTION GLUTATHIONE, P251

[5] SACCHAROMYCES-CEREVISIAE HAS AN INDUCIBLE RESPONSE TO MENADIONE WHICH DIFFERS FROM THAT TO HYDROGEN-PEROXIDE [J].

FLATTERYOBRIEN, J ;

COLLINSON, LP ;

DAWES, IW .

JOURNAL OF GENERAL MICROBIOLOGY, 1993, 139 :501-507

[6] GLUTATHIONE IN ESCHERICHIA-COLI IS DISPENSABLE FOR RESISTANCE TO H2O2 AND GAMMA-RADIATION [J].

GREENBERG, JT ;

DEMPLE, B .

JOURNAL OF BACTERIOLOGY, 1986, 168 (02) :1026-1029

[7]

HANGEARONIS R, 1993, CELL, V72, P165

[8]

INOUE M, 1989, GLUTATHIONE CHEM BIO, P613

[9] INDUCTION OF GLUTATHIONE-PEROXIDASE BY LINOLEIC-ACID HYDROPEROXIDE IN HANSENULA-MRAKII [J].

INOUE, Y ;

ICHIRYU, T ;

YOSHIKAWA, K ;

TRAN, LT ;

MURATA, K ;

KIMURA, A .

AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 1990, 54 (12) :3289-3293

[10]

INOUE Y, 1995, IN PRESS ADV MICROBI

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