BIOSYNTHESIS OF 5 MAIN HISTONE FRACTIONS OF RAT THYMUS

被引：28

作者：

DICK, C

JOHNS, EW

机构：

[1] Chester Beatty Research Institute, Institute of Cancer Research, Royal Cancer Hospital, London

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1969年 / 174卷 / 01期

基金：

英国医学研究理事会;

关键词：

D O I：

10.1016/0005-2787(69)90263-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The five main histone fractions, F1, F2(a)1, F2(a)2, F2(b) and F3, have been isolated from rat thymus 24 h after injection with l-[3H]lysine. These fractions have all been characterized by amino acid analyses and by polyacrylamide gel electrophoresis. Determination of relative specific activities showed that the rate of synthesis of each fraction was similar, variations occurring only if the fractions were impure. A method is also described which permits determination of relative specific activities directly from histone fractions on polyacrylamide gel. © 1969.

引用

页码：380 / +

页数：1

共 21 条

[1]

ALLFREY VG, 1966, HISTONES CIBA F STUD, V24, P42

[2]

BUSCH H, 1965, HISTONES OTHER NUCLE

[3] METABOLIC INTEGRITY OF DEOXYRIBONUCLEOHISTONES [J].