USE OF CO-60-EDTA AS AN EXTRACELLULAR MARKER IN FROG-SKIN

被引：8

作者：

FERREIRA, KTG

SWENSSON, WM

机构：

[1] Grupo de Biofísica, Centro de Biologia, Instituto Gulbenkian de Ciência, Oeiras

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1979年 / 552卷 / 01期

关键词：

!sup]60[!/sup]Co-EDTA; Electrolyte content; Extracellular space; Frog skin;

D O I：

10.1016/0005-2736(79)90256-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

60Co-EDTA was tested as an extracellular marker in isolated epithelia of frog skin. It was found to be non-toxic for frog skin and 0.1 mM EDTA proved to be enough to saturate any adsorption sites. Comparing with [14C]inulin, 60Co-EDTA marks a slightly greater extracellular space and this volume is constant between 15 min and 2 h. Furthermore it is reproducible and its use simplifies the methodology of the determination of water and electrolyte contents in the cells. © 1979.

引用

页码：178 / 182

页数：5

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