MAPPING OF RIPENING-RELATED OR RIPENING-SPECIFIC CDNA CLONES OF TOMATO (LYCOPERSICON-ESCULENTUM)

被引:44
作者
KINZER, SM [1 ]
SCHWAGER, SJ [1 ]
MUTSCHLER, MA [1 ]
机构
[1] CORNELL UNIV,DEPT PLANT BREEDING & BIOMETRY,252 EMERSON HALL,ITHACA,NY 14853
关键词
alc; Clustering; nor; RFLPs;
D O I
10.1007/BF00226158
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
Nineteen ripening-related or -specific clones from Lycopersicon esculentum were mapped via RFLP analysis using an F2 population from the cross L. esculentum x L. pennellii and cDNA or genomic clones of known map location. The map produced using cDNA and genomic clones of known map location corresponded well with previously published maps of tomato. The number of loci detected for each ripening-related or-specific clone varied from one to seven. These loci were located on all 12 chromosomes of the tomato genome. There was no significant clustering of ripening-related or-specific genes. Regions of very low recombination were observed. The clone for polygalacturonase (TOM6) mapped to a single region on chromosome 10, the same chromosome as the nor and alc ripening mutants. To fine map this chromosome, two backcross populations were produced from the cross of L. esculentum x L. pimpenillifolium, in which the esculentum parents used were homozygous for either the alc or the nor. The coding region for polygalacturonase is functionally unlinked to either of these two ripening mutants. © 1990 Springer-Verlag.
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页码:489 / 496
页数:8
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