MONOSIZED ADSORBENTS FOR HIGH-PERFORMANCE AFFINITY-CHROMATOGRAPHY - APPLICATION TO THE PURIFICATION OF CALF INTESTINAL ALKALINE-PHOSPHATASE AND HUMAN URINE UROKINASE

被引：18

作者：

CLONIS, YD ^{[1
]}

LOWE, CR ^{[1
]}

机构：

[1] UNIV CAMBRIDGE,INST BIOTECHNOL,TENNIS COURT RD,CAMBRIDGE CB2 1QT,ENGLAND

来源：

JOURNAL OF CHROMATOGRAPHY | 1991年 / 540卷 / 1-2期

关键词：

D O I：

10.1016/S0021-9673(01)88801-4

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Affinity absorbents comprising monodisperse spherical synthetic macroporous beads offer the propect of high-capacity, high-resolution separation of proteins at low operating pressures. Purpose-designed biomimetic dyes were covalently attached to Dynospheres XP-3507 beads and exploited for the purification of calf intestine alkaline phosphatease and human urine urokinase from crude extracts. This study demonstrates that the combination of specifically designed affinity ligands with monosized support materials is a powerful approach to the resolution of proteins by high-performance affinity chromatography.

引用

页码：103 / 111

页数：9

共 18 条

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[J]. BIOTECHNOLOGY AND BIOENGINEERING, 1987, 30 (05) : 621 - 627
[5] LARGE-SCALE AFFINITY-CHROMATOGRAPHY
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[9] CLONIS YD, 1990, SEPARATION PROCESSES, P401
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