CLONING AND SEQUENCE-ANALYSIS OF GYRA GENE OF KLEBSIELLA-PNEUMONIAE

被引：50

作者：

DIMRI, GP ^{[1
]}

DAS, HK ^{[1
]}

机构：

[1] JAWAHARLAL NEHRU UNIV, CTR BIOTECHNOL, GENET ENGN UNIT, NEW DELHI 110067, INDIA

来源：

NUCLEIC ACIDS RESEARCH | 1990年 / 18卷 / 01期

关键词：

D O I：

10.1093/nar/18.1.151

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The gene gyrA encoding the DNA gyrase A subunit of Klebsiella pneumoniae has been cloned in the plasmid pBR322. Bases of about 3.5 Kb DNA have been sequenced to locate the gyrA gene. An open reading frame of 2628 nucleotides coding for a 97 KD protein has been identified. Homology to the extent of about 85% was detected at the nucleotide level and about 90% at the amino acid level, when the sequences were compared with that of Escherichia coli gyrA. Some very interesting differences have, however, been found in the promoter region. © 1990 Oxford University Press.

引用

页码：151 / 156

页数：6

共 32 条

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