A HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY METHOD FOR SEPARATION OF PURINE-BASES, NUCLEOSIDES AND UREIDES - APPLICATION TO STUDIES ON PURINE CATABOLISM IN HIGHER-PLANTS

被引：14

作者：

ASHIHARA, H

YABUKI, N

MITSUI, K

机构：

[1] Department of Biology, Faculty of Science, Ochanomizu University, Tokyo

来源：

JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS | 1990年 / 21卷 / 01期

关键词：

HPLC; Purine basis; Purine catabolism; Purine nucleoside; Ureide; uric acid;

D O I：

10.1016/0165-022X(90)90045-E

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Mixtures of purine nucleotides, nucleosides, nucleobases, uric acid, allantoin and allantoic acid were fractionated by high-performance liquid chromatography on a polyvinyl alcohol gel column. Asahipak GS-320H, with isocratic elution by sodium phosphate. Application of this system to the determination of the sizes of cellular pools of purine derivatives in plant cells and of the activity of related enzymes, as well as to the purificaion of enzymatically synthesized radioactive compounds, is described. © 1990.

引用

页码：59 / 63

页数：5

共 11 条

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