A NOVEL SPERMIDINE-DEPENDENT ENDORIBONUCLEASE ACTIVITY CAUSED BY RNA-PROTEIN COMPLEX IN MOUSE FM3A CELL-EXTRACTS

被引：10

作者：

NASHIMOTO, M ^{[1
]}

KOMINAMI, R ^{[1
]}

NISHI, S ^{[1
]}

MISHIMA, Y ^{[1
]}

机构：

[1] NIIGATA UNIV,SCH MED,DEPT BIOCHEM,NIIGATA 95021,JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1991年 / 176卷 / 03期

关键词：

D O I：

10.1016/0006-291X(91)90407-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have found a novel spermidine-dependent endoribonuclease activity in mouse FM3A cell extracts. This endoribonuclease cleaves RNA substrates containing a sequence CCCCCGGUUUGU in its middle. This activity is lost either by heat- or micrococcal nuclease-pretreatment. When heat-pretreated extracts and micrococcal nuclease-pretreated ones are mixed, the activity is restored, suggesting that this activity requires both RNA and protein components. Testing the restoration of the lost endoribonuclease activity in micrococcal nuclease-pretreated extracts by addition of fractionated cellular RNAs, we identified an approximately 65 nucleotide RNA required for this endoribonuclease activity. © 1991.

引用

页码：1163 / 1169

页数：7

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