ANATOMICAL MAPPING OF CHOLINE-ACETYLTRANSFERASE (CHAT)-LIKE AND GLUTAMATE-DECARBOXYLASE (GAD)-LIKE IMMUNOREACTIVITY IN OUTER HAIR CELL EFFERENTS IN ADULT-RATS

被引：23

作者：

DANNHOF, BJ ^{[1
]}

ROTH, B ^{[1
]}

BRUNS, V ^{[1
]}

机构：

[1] JOHANN WOLFGANG GOETHE UNIV,INST ZOOL,SIESMAYERSTR 70,W-6000 FRANKFURT 11,GERMANY

来源：

CELL AND TISSUE RESEARCH | 1991年 / 266卷 / 01期

关键词：

COCHLEAR EFFERENTS; IMMUNOELECTRON MICROSCOPY; CHOLINE ACETYLTRANSFERASE; GLUTAMATE DECARBOXYLASE; RAT (WISTAR);

D O I：

10.1007/BF00678715

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The distribution of choline acetyltransferase (ChAT)-like and glutamate decarboxylase (GAD)-like immunoreactivity in the cochleae of 15 adult Wistar white rats was investigated using the peroxidase-antiperoxidase (PAP) technique. A monoclonal antibody to ChAT and a polyclonal antiserum to GAD were used. Immunoreaction was investigated quantitatively, in the electron microscope, on tangential sections of the tunnel of Corti and the rows of outer hair cells. ChAT-like and GAD-like immunoreactivity was found in all efferent nerve fibres in the tunnel of Corti and in all efferent synapses on the outer hair cells. A coexistence of ChAT and GAD in the efferent system to the outer hair cells of the rat is therefore assumed.

引用

页码：89 / 95

页数：7

共 35 条

[1]

Altschuler R. A., 1986, NEUROBIOLOGY HEARING, P161

[2] IMMUNOCYTOCHEMICAL LOCALIZATION OF CHOLINE ACETYLTRANSFERASE-LIKE IMMUNOREACTIVITY IN THE GUINEA-PIG COCHLEA [J].

ALTSCHULER, RA ;

KACHAR, B ;

RUBIO, JA ;

PARAKKAL, MH ;

FEX, J .

BRAIN RESEARCH, 1985, 338 (01) :1-11

[3] MORPHOLOGY OF LABELED EFFERENT FIBERS IN THE GUINEA-PIG COCHLEA [J].

BROWN, MC .

JOURNAL OF COMPARATIVE NEUROLOGY, 1987, 260 (04) :605-618

[4] EVOKED MECHANICAL RESPONSES OF ISOLATED COCHLEAR OUTER HAIR-CELLS [J].

BROWNELL, WE ;

BADER, CR ;

BERTRAND, D ;

DERIBAUPIERRE, Y .

SCIENCE, 1985, 227 (4683) :194-196

[5]

DAVIES ED, 1985, AUDITORY BIOCH, P244

[6]

EYBALIN M, 1987, EXP BRAIN RES, V65, P261

[7]

EYBALIN M, 1985, CR ACAD SCI III-VIE, V301, P679

[8] IMMUNOELECTRON MICROSCOPY IDENTIFIES SEVERAL TYPES OF GABA-CONTAINING EFFERENT SYNAPSES IN THE GUINEA-PIG ORGAN OF CORTI [J].

EYBALIN, M ;

PARNAUD, C ;

GEFFARD, M ;

PUJOL, R .

NEUROSCIENCE, 1988, 24 (01) :29-38

[9] IMMUNOELECTRON MICROSCOPIC LOCALIZATION OF NEUROTRANSMITTERS IN THE COCHLEA [J].

EYBALIN, M ;

ALTSCHULER, RA .

JOURNAL OF ELECTRON MICROSCOPY TECHNIQUE, 1990, 15 (03) :209-224

[10] GLUTAMIC-ACID DECARBOXYLASE IMMUNOREACTIVITY OF OLIVOCOCHLEAR NEURONS IN THE ORGAN OF CORTI OF GUINEA-PIG AND RAT [J].

FEX, J ;

ALTSCHULER, RA .

HEARING RESEARCH, 1984, 15 (02) :123-131

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