MEASUREMENT OF THE CONTENT OF LIMIT-DEXTRINASE IN CEREAL FLOURS

被引：57

作者：

MCCLEARY, BV

机构：

[1] MegaZyme (Australia) Pty. Ltd., North Rocks, NSW 2151

来源：

CARBOHYDRATE RESEARCH | 1992年 / 227卷

关键词：

D O I：

10.1016/0008-6215(92)85076-C

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Procedures for the quantitative extraction, activation, and assay of limit-dextrinase in cereal flours have been developed. Extraction and activation require incubation in buffer containing 20mM cysteine for at least 16 h or with 25mM dithiothreitol for 5 h. Activity is assayed with a soluble, dyed substrate (Red-Pullulan) or an insoluble, dyed, and cross-linked substrate (Azurine-CL-Pullulan) which is dispensed in tablet form (Limit-DextriZyme tablets).

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页码：257 / 268

页数：12

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