IMPROVING PURIFICATION OF RECOMBINANT RIBONUCLEASE-T1

被引：24

作者：

LANDT, O ^{[1
]}

ZIRPELGIESEBRECHT, M ^{[1
]}

MILDE, A ^{[1
]}

HAHN, U ^{[1
]}

机构：

[1] FREE UNIV BERLIN,INST KRISTALLOG,SAENGER ABT,TAKUSTR 6,W-1000 BERLIN 33,GERMANY

来源：

JOURNAL OF BIOTECHNOLOGY | 1992年 / 24卷 / 02期

关键词：

RECOMBINANT RIBONUCLEASE-T1; ESCHERICHIA-COLI; PROTEIN ENGINEERING; FPLC;

D O I：

10.1016/0168-1656(92)90122-P

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Purification of recombinant RNase T1 and its mutants has been improved by optimizing bacterial growth conditions, periplasmic fraction preparation and the use of a precolumn. The main part of the chromatographic separation could be automated due to the reproducibility of the procedure.

引用

页码：189 / 194

页数：6

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