DETERMINATION OF THE DNA-BINDING SITE OF THE GAL4 PROTEIN - A PHOTO-CIDNP STUDY

被引：3

作者：

SERIKAWA, Y

SHIRAKAWA, M

KYOGOKU, Y

机构：

[1] Institute for Protein Research, Osaka University, Suita, Osaka

来源：

FEBS LETTERS | 1992年 / 299卷 / 03期

关键词：

NMR; PHOTO-CIDNP; GAL4; UASG; ZINC FINGER; DNA BINDING;

D O I：

10.1016/0014-5793(92)80115-W

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

After the assignment of H-1 NMR signals of aromatic side chains by means of specific deuteration, we analyzed the DNA binding site of GAL4 by measuring photo-CIDNP spectra. The results showed that Trp36 is involved in both the specific interaction with UAS(G) and non-specific DNA binding. This residue is located inside the Cys-rich region, but outside the putative Zn-finger. The photo-CIDNP spectrum also showed that the side chains of Tyr40 and His53 are not exposed on the surface of the protein.

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页码：205 / 208

页数：4

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