STABILIZATION OF BACTERIOPHAGE MU-REPRESSOR-OPERATOR COMPLEXES BY THE ESCHERICHIA-COLI INTEGRATION HOST FACTOR PROTEIN

被引:29
作者
GAMA, MJ
TOUSSAINT, A
HIGGINS, NP
机构
[1] UNIV LIBRE BRUXELLES,GENET LAB,UNITE TRANSPOSIT BACTERIENNE,B-1640 RHODE ST GENESE,BELGIUM
[2] UNIV ALABAMA,DEPT BIOCHEM,BIRMINGHAM,AL 35294
关键词
D O I
10.1111/j.1365-2958.1992.tb00896.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
All of the previously described effects of integration host factor (IHF) on bacteriophage Mu development have supported the view that IHF favours transposition-replication over the alternative state of lysogenic phage growth. In this report we show that, consistent with a model in which Mu repressor binding to its operators requires a particular topology of the operator DNA, IHF stimulates repressor binding to the O1 and O2 operators and enhances Mu repression. IHF would thus be one of the keys, besides supercoiling and the H-NS protein, that lock the operator region into the appropriate topological conformation for high-affinity binding not only of the phage transposase but also of the phage repressor.
引用
收藏
页码:1715 / 1722
页数:8
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