CHARACTERIZATION OF CALLUS FORMATION AND CAMPTOTHECIN PRODUCTION BY CELL-LINES OF CAMPTOTHECA-ACUMINATA

被引：68

作者：

VANHENGEL, AJ ^{[1
]}

HARKES, MP ^{[1
]}

WICHERS, HJ ^{[1
]}

HESSELINK, PGM ^{[1
]}

BUITELAAR, RM ^{[1
]}

机构：

[1] TNO NUTR & FOOD RES,DEPT BIOTECHNOL,POB 360,3700 AJ ZEIST,NETHERLANDS

来源：

PLANT CELL TISSUE AND ORGAN CULTURE | 1992年 / 28卷 / 01期

关键词：

ANTITUMOR ACTIVITY; CAMPTOTHECA-ACUMINATA; CAMPTOTHECIN; PLANT CELL CULTURE; SECONDARY METABOLISM;

D O I：

10.1007/BF00039910

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Camptotheca acuminata plants contain camptothecin which is a secondary metabolite with strong anti-tumor activity. The induction of callus and cell suspension cultures from Camptotheca acuminata stem parts on different media is described. Growth in the original media and in media with different salts and/or vitamins was followed by measuring several growth parameters. Camptothecin was detected and identified by means of TLC, HPLC and GC-MS. The production,of camptothecin in cell suspension cultures was followed and compared to the original plant material. Results indicate that high density cultures could be easily established, producing ca 1 mg l-1 of camptothecin.

引用

页码：11 / 18

页数：8

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