PURPALD (4-AMINO-3-HYDRAZINO-5-MERCAPTO-1,2,4-TRIAZOLE) AS A REAGENT FOR POSTCOLUMN DERIVATIZATION OF NEUTRAL MONOSACCHARIDES IN HIGH-PRESSURE LIQUID-CHROMATOGRAPHY

被引：10

作者：

DELNOZAL, MJ ^{[1
]}

BERNAL, JL ^{[1
]}

HERNANDEZ, V ^{[1
]}

TORIBIO, L ^{[1
]}

MENDEZ, R ^{[1
]}

机构：

[1] UNIV LEON,FAC BIOL SCI,DEPT BIOCHEM & MOLEC BIOL,E-24071 LEON,SPAIN

来源：

JOURNAL OF LIQUID CHROMATOGRAPHY | 1993年 / 16卷 / 05期

关键词：

D O I：

10.1080/10826079308019574

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A comprehensive study of the reaction between Purpald (4-amino-3- hydrazino-5-mercapto-1,2,4-triazole) and eight neutral monosaccharides in order to establish the optimal conditions (wavelength, oxygen supply, temperature, time, pH and concentration range) was carried out. The reaction was applied to the post-column detection in HPLC of monosaccharides eluted with water from an ion-exchange column. The detection limits and linear determination ranges thus achieved make this reaction a valid alternative to the determination of carbohydrates.

引用

页码：1105 / 1116

页数：12