COMPARISON OF COVALENT AND NONCOVALENT LABELING WITH NEAR-INFRARED DYES FOR THE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF HUMAN SERUM-ALBUMIN

被引:87
作者
WILLIAMS, RJ [1 ]
LIPOWSKA, M [1 ]
PATONAY, G [1 ]
STREKOWSKI, L [1 ]
机构
[1] GEORGIA STATE UNIV,DEPT CHEM,ATLANTA,GA 30303
关键词
D O I
10.1021/ac00053a019
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Noncovalent and covalent methods of labeling protein with near-infrared polymethine cyanine dyes were compared for use In analyzing human serum albumin (HSA) by high-performance liquid chromatography (HPLC) with near-infrared absorbance detection. While noncovalent labeling was faster than covelent labeling and took place in the physiological pH range, covalent labeling was more stable under conditions encountered in many of the widely used types of HPLC. Covalently labeled HSA protein peaks indicated uniform labeling of amino groups at both hydrophilic and hydrophobic binding sites, while noncovalent labeling showed a preference for hydrophobic binding sites.
引用
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页码:601 / 605
页数:5
相关论文
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