DETAILED STRUCTURAL CHARACTERIZATION OF SUCCINOGLYCAN, THE MAJOR EXOPOLYSACCHARIDE OF RHIZOBIUM-MELILOTI RM1021

被引：147

作者：

REINHOLD, BB ^{[1
]}

CHAN, SY ^{[1
]}

REUBER, TL ^{[1
]}

MARRA, A ^{[1
]}

WALKER, GC ^{[1
]}

REINHOLD, VN ^{[1
]}

机构：

[1] MIT,DEPT BIOL,CAMBRIDGE,MA 02139

来源：

JOURNAL OF BACTERIOLOGY | 1994年 / 176卷 / 07期

关键词：

D O I：

10.1128/jb.176.7.1997-2002.1994

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The detailed structure of the symbiotically important exopolysaccharide succinoglycan from Rhizobium meliloti Rm1021 was determined by mass spectrometry with electrospray ionization and collision-induced dissociation of the octameric oligosaccharide repeating unit. Previously undetermined locations of the succinyl and acetyl modifications were determined, in respect to both the residue locations within the octamer and the carbon positions within the pyranose ring. Glycosidic linkages determined previously by methylation analysis were also verified.

引用

收藏

页码：1997 / 2002

页数：6

相关论文

共 20 条

[1] HOST-SYMBIONT INTERACTIONS .4. STRUCTURAL ELUCIDATION, USING HPLC-MS AND GLC-MS OF THE ACIDIC POLYSACCHARIDE SECRETED BY RHIZOBIUM-MELILOTI STRAIN-1021 [J].

AMAN, P ;

MCNEIL, M ;

FRANZEN, LE ;

DARVILL, AG ;

ALBERSHEIM, P .

CARBOHYDRATE RESEARCH, 1981, 95 (02) :263-282

[2] PURIFICATION AND PROPERTIES OF A SPECIFIC, INDUCIBLE BETA-GLUCANASE, SUCCINOGLUCAN DEPOLYMERASE FROM FLAVOBACTERIUM [J].

AMEMURA, A ;

MOORI, K ;

HARADA, T .

BIOCHIMICA ET BIOPHYSICA ACTA, 1974, 334 (02) :398-409

[3] STUDIES ON AN EXTRACELLULAR POLYSACCHARIDE FROM RHIZOBIUM-MELILOTI [J].

BJORNDAL, H ;

ERBING, C ;

LINDBERG, B ;

FAHRAEUS, G ;

LJUNGGREN, H .

ACTA CHEMICA SCANDINAVICA, 1971, 25 (04) :1281-+

[4]

BJORNDAL H, 1970, ANGEW CHEM, V82, P643

[5] A SIMPLE AND RAPID METHOD FOR THE PERMETHYLATION OF CARBOHYDRATES [J].

CIUCANU, I ;

KEREK, F .

CARBOHYDRATE RESEARCH, 1984, 131 (02) :209-217

[6] A SYSTEMATIC NOMENCLATURE FOR CARBOHYDRATE FRAGMENTATIONS IN FAB-MS MS SPECTRA OF GLYCOCONJUGATES [J].

DOMON, B ;

COSTELLO, CE .

GLYCOCONJUGATE JOURNAL, 1988, 5 (04) :397-409

[7] ELECTROSPRAY IONIZATION FOR MASS-SPECTROMETRY OF LARGE BIOMOLECULES [J].

FENN, JB ;

MANN, M ;

MENG, CK ;

WONG, SF ;

WHITEHOUSE, CM .

SCIENCE, 1989, 246 (4926) :64-71

[8] METHYLATION ANALYSIS OF COMPLEX CARBOHYDRATES IN SMALL AMOUNTS - CAPILLARY GAS CHROMATOGRAPHY-MASS FRAGMENTOGRAPHY OF METHYLALDITOL ACETATES OBTAINED FROM N-GLYCOSIDICALLY LINKED GLYCOPROTEIN OLIGOSACCHARIDES [J].

GEYER, R ;

GEYER, H ;

KUHNHARDT, S ;

MINK, W ;

STIRM, S .

ANALYTICAL BIOCHEMISTRY, 1983, 133 (01) :197-207

[9] A NOVEL EXOPOLYSACCHARIDE CAN FUNCTION IN PLACE OF THE CALCOFLUOR-BINDING EXOPOLYSACCHARIDE IN NODULATION OF ALFALFA BY RHIZOBIUM-MELILOTI [J].

GLAZEBROOK, J ;

WALKER, GC .

CELL, 1989, 56 (04) :661-672

[10] STRUCTURAL STUDIES OF A NOVEL EXOPOLYSACCHARIDE PRODUCED BY A MUTANT OF RHIZOBIUM-MELILOTI STRAIN RM1021 [J].

HER, GR ;

GLAZEBROOK, J ;

WALKER, GC ;

REINHOLD, VN .

CARBOHYDRATE RESEARCH, 1990, 198 (02) :305-312