CONTROL OF ATP-DEPENDENT CO2 FIXATION IN EXTRACTS OF HYDROGENOMONAS FACILIS - NADH REGULATION OF PHOSPHORIBULOKINASE

被引：27

作者：

MACELROY, RD

JOHNSON, EJ

JOHNSON, MK

机构：

[1] Exobiology Division, Ames Research Center, NASA, Moffett Field

来源：

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS | 1969年 / 131卷 / 01期

基金：

美国国家科学基金会;

关键词：

D O I：

10.1016/0003-9861(69)90131-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The role of NADH in stimulating ATP-dependent CO2 fixation has been examined in crude extracts of Hydrogenomonas facilis. It has been found that the stimulation cannot be attributed to reduction of the product, 3-phosphoglyceric acid, and consequent regeneration of substrate. Rather, NADH appears to specifically affect phosphoribulokinase, probably by causing an allosteric transition of this regulatory enzyme. The kinetics of the NADH effect on CO2 fixation suggest cooperative binding of the effector. A similar cooperative response of the enzyme to ATP has been noted. It is suggested that the control of CO2 fixation is mediated by allosteric regulation of phosphoribulokinase activity. © 1969.

引用

页码：272 / &

共 8 条

[1]

AXELROD B, 1954, J BIOL CHEM, V209, P847

[2]

DAVIS DH, 1967, THESIS U CALIFORNIA

[3] OCCURRENCE OF ADENOSINE MONOPHOSPHATE INHIBITION OF CARBON DIOXIDE FIXATION IN PHOTOSYNTHETIC AND CHEMOSYNTHETIC AUTOTROPHS [J].