MAPPING ANTI-MULLERIAN HORMONE (AMH) AND RELATED SEQUENCES IN THE MOUSE - IDENTIFICATION OF A NEW REGION OF HOMOLOGY BETWEEN MMU10 AND HSA19P

A panel of 78 backcross progeny, BALB cJ × ( BALB cJ × CAST Ei)F1 was used to map the gene encoding anti-Müllerian hormone (Amh), also called Müllerian inhibiting substance, to mouse Chromosome 10 (MMU10). This analysis identified a new region of linkage homology between human Chromosome 19p (HSA 19p) and MMU10 and localized an apparent recombinational hot spot in ( C57BL 6J × Mus spretus)F1 females [compared with ( BALB cJ × CAST Ei)F1 males] to the interval between phenylalanine hydroxylase (Pah) and mast cell growth factor (Mgf). In addition, eight unlinked polymorphic sequences, provisionally designated Amh-related sequences (Amh-rs1 through Amh-rs8), were identified by Southern blot analysis using Amh probes. Amh-rs1, -rs2, -rs4, and -rs7 were mapped to MMU1, 13, 12, and 15, respectively, by recombinant inbred (RI) strain and intraspecific backcross analyses. The NXSM RI strain distribution patterns for the four unmapped loci are also presented. © 1991.