A RAPID METHOD FOR STARTING A CULTURE FOR THE ESTABLISHMENT OF EPSTEIN-BARR VIRUS-TRANSFORMED HUMAN LYMPHOBLASTOID CELL-LINES

被引：24

作者：

FUKUSHIMA, Y

OHASHI, H

WAKUI, K

NISHIDA, T

OHISHI, T

机构：

[1] SAITAMA CHILDRENS MED CTR,DEPT CLIN LAB,IWATSUKI,SAITAMA 339,JAPAN

[2] SAITAMA CHILDRENS MED CTR,DIV INFECT DIS IMMUNOL & ALLERGOL,IWATSUKI,SAITAMA 339,JAPAN

来源：

JAPANESE JOURNAL OF HUMAN GENETICS | 1992年 / 37卷 / 02期

关键词：

CELL CULTURE; EB VIRUS-TRANSFORMED LYMPHOBLASTOID CELL LINES; A RAPID METHOD;

D O I：

10.1007/BF01899737

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

We developed a simple and efficient procedure for the establishment of Epstein-Barr virus-transformed human lymphoblastoid cell lines. B-lymphocytes were obtained by centrifugation after hemolysis of red cells with a hemolysis buffer, instead of Ficoll-Parque gradient. We can start a primary culture within 15 min by using this method.

引用

页码：149 / 150

页数：2