COMPLEMENT GENE-EXPRESSION IN NEUROBLASTOMA AND ASTROCYTOMA CELL-LINES OF HUMAN-ORIGIN

被引：34

作者：

WALKER, DG

MCGEER, PL

机构：

[1] Kinsmen Laboratory of Neurological Research, Department of Psychiatry, University of British Columbia, Vancouver, BC

来源：

NEUROSCIENCE LETTERS | 1993年 / 157卷 / 01期

关键词：

COMPLEMENT; NEUROBLASTOMA; ASTROCYTOMA; POLYMERASE CHAIN REACTION; ALZHEIMER DISEASE;

D O I：

10.1016/0304-3940(93)90652-2

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Activation of the complement system is believed to be involved in degenerative processes of certain neurological diseases, including Alzheimer disease. Recent data have shown that the mRNAs for these proteins can be detected in brain-derived mRNA. In this study, C4 mRNA was detected by polymerase chain reaction (PCR) amplification of mRNA from the human neuroblastoma cell lines IMR32. SK-SH and SK-MC, and the human astrocytoma cell line U373MG, while C3 expression was detected in SK-MC, SK-SH and U373MG cells. The SK-MC and U373MG cells expressed mRNA for C9. The mRNA for C1qB could not be detected in any of these cell lines.

引用

页码：99 / 102

页数：4

共 20 条

[1] INTERFERON-GAMMA REGULATION OF C3-GENE EXPRESSION IN HUMAN ASTROGLIOMA CELLS [J].

BARNUM, SR ;

JONES, JL ;

BENVENISTE, EN .

JOURNAL OF NEUROIMMUNOLOGY, 1992, 38 (03) :275-282

[2]

BELT KT, 1984, CELL, V36, P907

[3]

CHOMCZYNSKI P, 1987, ANAL BIOCHEM, V162, P156, DOI 10.1016/0003-2697(87)90021-2

[4]

DEBRUIJN MHL, 1985, P NATL ACAD SCI USA, V82, P708

[5] NUCLEOTIDE-SEQUENCE OF CDNA AND DERIVED AMINO-ACID-SEQUENCE OF HUMAN-COMPLEMENT COMPONENT-C9 [J].

DISCIPIO, RG ;

GEHRING, MR ;

PODACK, ER ;

KAN, CC ;

HUGLI, TE ;

FEY, GH .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1984, 81 (23) :7298-7302

[6]

EIKELENBOOM P, 1989, VIRCHOWS ARCH B, V56, P259

[7]

ERCOLANI L, 1988, J BIOL CHEM, V263, P15335

[8] THE ROLE OF COMPLEMENT IN INFLAMMATION AND PHAGOCYTOSIS [J].

FRANK, MM ;

FRIES, LF .

IMMUNOLOGY TODAY, 1991, 12 (09) :322-326

[9] DETECTION OF COMPLEMENT PROTEIN MESSENGER-RNA IN HUMAN ASTROCYTES BY THE POLYMERASE CHAIN-REACTION [J].

GORDON, DL ;

AVERY, VM ;

ADRIAN, DL ;

SADLON, TA .

JOURNAL OF NEUROSCIENCE METHODS, 1992, 45 (03) :191-197

[10] SYNTHETIC ALZHEIMER AMYLOID BETA-A4 PEPTIDES ENHANCE PRODUCTION OF COMPLEMENT C3 COMPONENT BY CULTURED MICROGLIAL CELLS [J].

HAGA, S ;

IKEDA, K ;

SATO, M ;

ISHII, T .

BRAIN RESEARCH, 1993, 601 (1-2) :88-94

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