DNAK-MEDIATED ALTERATIONS IN HUMAN GROWTH-HORMONE PROTEIN INCLUSION-BODIES

被引：73

作者：

BLUM, P

VELLIGAN, M

LIN, N

MATIN, A

机构：

[1] STANFORD UNIV,MED CTR,SCH MED,DEPT MICROBIOL & IMMUNOL,STANFORD,CA 94305

[2] GENENTECH INC,SAN FRANCISCO,CA 94080

来源：

BIO-TECHNOLOGY | 1992年 / 10卷 / 03期

关键词：

D O I：

10.1038/nbt0392-301

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Protein overproduction in microbes frequently results in protein misfolding and aggregation though the molecular basis for this process is unclear. The HSP70 chaperonin, DnaK, was identified as an important factor controlling heterologous protein aggregation in Escherichia coli. Co-overproduction of DnaK significantly reduced human growth hormone (HGH) protein inclusion body formation and the extent of HGH aggregation.

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页码：301 / 304

页数：4

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