BEHAVIOR OF PERIOD-ALTERED CIRCADIAN-RHYTHM MUTANTS OF DROSOPHILA IN LIGHT - DARK CYCLES (DIPTERA, DROSOPHILIDAE)

被引:157
作者
HAMBLENCOYLE, MJ [1 ]
WHEELER, DA [1 ]
RUTILA, JE [1 ]
ROSBASH, M [1 ]
HALL, JC [1 ]
机构
[1] BRANDEIS UNIV,DEPT BIOL,235 BASSINE BLDG,WALTHAM,MA 02254
关键词
LOCOMOTOR ACTIVITY; PER-SHORT MUTANT; PER-LONG MUTANTS; PER-TRANSGENICS; CLOCK MUTANT; BLIND NORPA MUTANT; PHASE ANALYSIS;
D O I
10.1007/BF01058189
中图分类号
Q96 [昆虫学];
学科分类号
摘要
Adults of Drosophila melanogaster had their locomotor activity monitored under conditions of cycling light and dark (12 h each per cycle). The elementary behavior of wild-type flies under these "LD" conditions fluctuated between levels of high and levels of low activity. Two high-activity peaks occurred within a given cycle: one at about dawn; the other, at around dusk. Such accentuated activity levels gradually subsided to troughs in the middle of the day and of the night, after which the flies anticipated the next environmental transition by gradually becoming more active. Descriptions of these activity profiles were augmented by newly developed formal analyses of the "diel rhythm " phases (based in part on digital filterings of the raw behavioral data). The applications of these analyses led to objective, automated determination of when in the morning and the evening the flies' activity peaks occur. This normal diel behavior was compared to the locomotor activity and phase determinations for a series of rhythm variants. Most of these involved mutations at the period (per) locus and germ-line transformants bearing normal or altered forms of DNA cloned from this "clock gene." Such genetic variants have been shown previously to exhibit, in constant darkness, strain-specific circadian periods ranging from about 19 to about 29 h. We now show that the phases of the evening peaks of activity under LD conditions were correspondingly earlier than normal for the short-period mutants and later than normal for those with long circadian cycle durations. The morning peaks, however, moved (in comparison to the normal phase position) minimally under the influence of a given per variant.
引用
收藏
页码:417 / 446
页数:30
相关论文
共 50 条
[1]  
Aschoff J., Freerunning and entrained circadian rhythms, Handbook of Behavioral Neurobiology, Vol.4: Biological Rhythms, pp. 81-93, (1981)
[2]  
Aschoff J., Pohl H., Phase relations between a circadian rhythm and its Zeitgeber within the range of entrainment, Naturwissenchaften, 65, pp. 80-84, (1978)
[3]  
Baylies M.K., Bargiello T.A., Jackson F.R., Young M.W., Changes in abundance and structure of the per gene product can alter periodicity of the Drosophila clock, Nature, 326, pp. 390-392, (1987)
[4]  
Citri Y., Colot H.V., Jacquier A.C., Yu Q., Hall J.C., Baltimore D., Rosbash M., A family of unusually spliced and biologically active transcripts encoded by a Drosophila clock gene, Nature, 326, pp. 42-47, (1987)
[5]  
Dowse H.B., Ringo J.M., The search for hidden periodicities in biological time series revisited, J. Theoret. Biol., 139, pp. 487-515, (1989)
[6]  
Dowse H.B., Ringo J.M., Is the circadian clock a “metaoscillator”? Evidence from studies of ultradian rhythms in Drosophila., Molecular Genetics of Biological Rhythms, (1992)
[7]  
Dushay M.S., Rosbash M., Hall J.C., The disconnected visual system mutations in Drosophila melanogaster drastically disrupt circadian rhythms, J. Biol. Rhythms, 4, pp. 1-27, (1989)
[8]  
Dushay M.S., Konopka R.J., Orr D., Greenacre M.L., Kyriacou C.P., Rosbash M., Hall J.C., Phenotypic and genetic analysis of Clock, a new circadian rhythm mutant in Drosophila melanogaster, Genetics, 125, pp. 557-578, (1990)
[9]  
Engelmann W., Mack J., Different oscillators control the circadian rhythm of eclosion and activity in Drosophila, J. Comp. Physiol., 127, pp. 229-237, (1978)
[10]  
Hall J.C., Genetics of circadian rhythms, Annu. Rev. Genet., 24, pp. 659-697, (1990)