PROLIFERATION OF CHICKEN MYOBLASTS IS REGULATED BY SPECIFIC ISOFORMS OF PLATELET-DERIVED GROWTH-FACTOR - EVIDENCE FOR DIFFERENCES BETWEEN MYOBLASTS FROM MID AND LATE STAGES OF EMBRYOGENESIS

被引:37
作者
YABLONKAREUVENI, Z [1 ]
SEIFERT, RA [1 ]
机构
[1] UNIV WASHINGTON,SCH MED,DEPT PATHOL,SEATTLE,WA 98195
关键词
D O I
10.1006/dbio.1993.1079
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
In the present study we measured the level of PDGF receptor expression by chicken myoblasts and the effect of the three different PDGF isoforms (AA, AB, BB) on DNA synthesis by myoblasts. We examined PDGF receptor expression and function on clonally derived myoblasts in order to eliminate contaminating fibroblasts which are present in myogenic cultures and which bind PDGF. Furthermore, since we have previously shown that fetal myoblasts are replaced with adult myoblasts during late chicken embryogenesis, we compared PDGF receptor expression and function on myoblasts from Embryonic Day 10 (E10, mid development) and from Embryonic Day 19 (E19, late development). We found that all myogenic clones from late embryos (E19) express many receptors for PDGF-BB, far fewer receptors for PDGF-AB, and even fewer, if any, receptors for PDGF-AA. Myoblast clones derived from E10 were more heterogeneous in their PDGF binding pattern ranging from clones similar to E19 clones to clones having very few PDGF binding sites. We also found that both PDGF-AB and PDGF-BB can promote DNA synthesis by clonally derived chicken myoblasts maintained in 2.5% fetal bovine serum whereas PDGF-AA has no detectable effect. Finally, we observed that primary myogenic cultures from E10 and E19 differ strikingly in levels of PDGF binding; E19 cultures bind much more PDGF than do E10 cultures. We conclude that PDGF can enhance the proliferation of chicken myoblasts and that myoblasts responsive to PDGF are more frequent in late than in mid stages of development. We propose that PDGF may be a modulator of myogenesis of adult but not fetal myoblasts. © 1993 by Academic Press, Inc.
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页码:307 / 318
页数:12
相关论文
共 62 条
  • [1] ALLEN RE, 1990, P SOC EXP BIOL MED, V194, P81
  • [2] IMMUNOCHEMICAL ANALYSIS OF MYOSIN HEAVY-CHAIN DURING AVIAN MYOGENESIS INVIVO AND INVITRO
    BADER, D
    MASAKI, T
    FISCHMAN, DA
    [J]. JOURNAL OF CELL BIOLOGY, 1982, 95 (03) : 763 - 770
  • [3] SPECIFICITY OF CHICKEN AND MAMMALIAN TRANSFERRINS IN MYOGENESIS
    BEACH, RL
    POPIELA, H
    FESTOFF, BW
    [J]. CELL DIFFERENTIATION, 1985, 16 (02): : 93 - 100
  • [4] PROLIFERATION OF MUSCLE SATELLITE CELLS ON INTACT MYOFIBERS IN CULTURE
    BISCHOFF, R
    [J]. DEVELOPMENTAL BIOLOGY, 1986, 115 (01) : 129 - 139
  • [5] BOWENPOPE DF, 1989, J BIOL CHEM, V264, P2502
  • [6] GROWTH-FACTOR CONTROL OF SKELETAL-MUSCLE DIFFERENTIATION - COMMITMENT TO TERMINAL DIFFERENTIATION OCCURS IN G1 PHASE AND IS REPRESSED BY FIBROBLAST GROWTH-FACTOR
    CLEGG, CH
    LINKHART, TA
    OLWIN, BB
    HAUSCHKA, SD
    [J]. JOURNAL OF CELL BIOLOGY, 1987, 105 (02) : 949 - 956
  • [7] SKELETAL-MUSCLE SATELLITE CELL DIVERSITY - SATELLITE CELLS FORM FIBERS OF DIFFERENT TYPES IN CELL-CULTURE
    FELDMAN, JL
    STOCKDALE, FE
    [J]. DEVELOPMENTAL BIOLOGY, 1991, 143 (02) : 320 - 334
  • [8] TEMPORAL APPEARANCE OF SATELLITE CELLS DURING MYOGENESIS
    FELDMAN, JL
    STOCKDALE, FE
    [J]. DEVELOPMENTAL BIOLOGY, 1992, 153 (02) : 217 - 226
  • [9] PLATELET-DERIVED GROWTH-FACTOR RECEPTORS IN THE KIDNEY - UPREGULATED EXPRESSION IN INFLAMMATION
    FELLSTROM, B
    KLARESKOG, L
    HELDIN, CH
    LARSSON, E
    RONNSTRAND, L
    TERRACIO, L
    TUFVESON, G
    WAHLBERG, J
    RUBIN, K
    [J]. KIDNEY INTERNATIONAL, 1989, 36 (06) : 1099 - 1102
  • [10] HORMONES, GROWTH-FACTORS, AND MYOGENIC DIFFERENTIATION
    FLORINI, JR
    EWTON, DZ
    MAGRI, KA
    [J]. ANNUAL REVIEW OF PHYSIOLOGY, 1991, 53 : 201 - 216