SUSCEPTIBILITY OF NONPRIMATE CELL-LINES TO HEPATITIS-A VIRUS-INFECTION

被引：39

作者：

DOTZAUER, A ^{[1
]}

FEINSTONE, SM ^{[1
]}

KAPLAN, G ^{[1
]}

机构：

[1] US FDA,CTR BIOL EVALUAT & RES,OFF VACCINES RES & REVIEW,DIV VIRAL PROD,HEPATITIS RES LAB,BETHESDA,MD 20892

来源：

JOURNAL OF VIROLOGY | 1994年 / 68卷 / 09期

关键词：

D O I：

10.1128/JVI.68.9.6064-6068.1994

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Hepatitis A virus (HAV) has been adapted to grow in primate cell cultures. We investigated replication of HAV in nonprimate cells by inoculating 20 cell lines from different species with the tissue culture-adapted HM175 strain. Slot blot hybridization and immunofluorescence analysis revealed that HAV replicated in GPE, SP 1K, and LB-RS-2 D10 cells of guinea pig, dolphin, and pig origin, respectively. Studies in IB-RS-2 D10 cells were discontinued because cultures were contaminated with classical swine fever virus. A growth curve showed that HAV grew poorly in GPE cells and intermediately in SP 1K cells compared with growth in FRhK-4 cells. Therefore, the cell surface receptor(s) and other host factor(s) required for HAV replication are present in nonprimate as well as primate cells.

引用

页码：6064 / 6068

页数：5

共 24 条

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