EFFECT OF VITAMIN-A ACID ON GROWTH OF KERATINOCYTES IN CULTURE

被引：13

作者：

WILKINSON, DI

机构：

[1] Department of Dermatology, Stanford University, School of Medicine, Stanford, 94305, California

来源：

ARCHIVES OF DERMATOLOGICAL RESEARCH | 1978年 / 263卷 / 01期

关键词：

DNA levels; Keratinocyte cell culture; Retinoic acid; Thymidine incorporation;

D O I：

10.1007/BF00446856

中图分类号：

R75 [皮肤病学与性病学];

学科分类号：

100206 ;

摘要：

The effect of vitamin A acid on the culture of guinea pig ear keratinocytes was investigated. Use of this agent and treatment of the plastic dishes with collagen solution facilitated attachment and growth. Vitamin A acid elicited a wave of DNA synthesis which was studied by incoporation of tritiated thymidine and quantitation of cellular DNA, particularly during the period 24-45 h after plating. During this period, thymidine incorporation into DNA was increased ten-fold by vitamin A acid and showed a complex pattern of peaks and shoulders which may represent the S phase of synchronous keratinocytes. © 1978 Springer-Verlag.

引用

页码：75 / 81

页数：7

共 18 条

[1] GROWTH STIMULATION OF CULTURED POSTEMBRYONIC EPIDERMAL-CELLS BY VITAMIN-A ACID [J].