2-DIMENSIONAL ELECTROPHORESIS OF SURFACE GLYCOPROTEINS OF NORMAL BHK CELLS AND RICIN RESISTANT MUTANTS

被引：26

作者：

PENA, SDJ ^{[1
]}

MILLS, G ^{[1
]}

HUGHES, RC ^{[1
]}

机构：

[1] NATL INST MED RES, LONDON NW7 1AA, ENGLAND

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1979年 / 550卷 / 01期

关键词：

(Fibroblast); 2-dimensional electrophoresis; Lactoperoxidase; Lectin-resistant mutant; Surface labelling;

D O I：

10.1016/0005-2736(79)90118-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The surface glycoproteins of baby hamster kidney (BHK) cells were iodinated by lactoperoxidase and submitted to a 2-dimensional electrophoresis procedure involving isoelectric focusing in the 1st dimension and SDS [sodium dodecyl sulfate] gel electrophoresis in the 2nd dimension. After autoradiography, a complex by reproducible pattern was obtained. The technique was then applied to the study of 3 ricin[Ricinus communis]-resistant mutant clones with reduced rates of cell-cell and/or cell-substratum adhesion. Abnormal patterns were observed in all 3 mutant clones, indicating different ricin resistance mechanisms and identifying glycoproteins which may be involved in cellular interactions.

引用

页码：100 / 109

页数：10

共 32 条

[1] LEVELS OF TRANSLATABLE MESSENGER-RNAS FOR CELL-SURFACE PROTEIN, COLLAGEN PRECURSORS, AND 2 MEMBRANE PROTEINS ARE ALTERED IN ROUS-SARCOMA VIRUS-TRANSFORMED CHICK-EMBRYO FIBROBLASTS [J].